Induction of cytotoxic T lymphocytes of heterogeneous specificities by immunization with a single peptide derived from influenza A virus.
Identifieur interne : 001903 ( Main/Exploration ); précédent : 001902; suivant : 001904Induction of cytotoxic T lymphocytes of heterogeneous specificities by immunization with a single peptide derived from influenza A virus.
Auteurs : H. Masaki [Japon] ; M. Tamura ; I. KuraneSource :
- Viral immunology [ 0882-8245 ] ; 2000.
Descripteurs français
- KwdFr :
- Activation des lymphocytes, Animaux, Complexe majeur d'histocompatibilité, Cytotoxicité immunologique, Femelle, Immunisation, Infections à Orthomyxoviridae (), Lymphocytes T cytotoxiques (immunologie), Peptides (administration et posologie), Peptides (immunologie), Phénotype, Protéines virales (immunologie), Réactions croisées, Souris, Souris de lignée BALB C, Virus de la grippe A (), Virus de la grippe A (immunologie).
- MESH :
- administration et posologie : Peptides.
- immunologie : Lymphocytes T cytotoxiques, Peptides, Protéines virales, Virus de la grippe A.
- Activation des lymphocytes, Animaux, Complexe majeur d'histocompatibilité, Cytotoxicité immunologique, Femelle, Immunisation, Infections à Orthomyxoviridae, Phénotype, Réactions croisées, Souris, Souris de lignée BALB C, Virus de la grippe A.
English descriptors
- KwdEn :
- Animals, Cross Reactions, Cytotoxicity, Immunologic, Female, Immunization, Influenza A virus (chemistry), Influenza A virus (immunology), Lymphocyte Activation, Major Histocompatibility Complex, Mice, Mice, Inbred BALB C, Orthomyxoviridae Infections (prevention & control), Peptides (administration & dosage), Peptides (immunology), Phenotype, T-Lymphocytes, Cytotoxic (immunology), Viral Proteins (immunology).
- MESH :
- chemical , administration & dosage : Peptides.
- chemistry : Influenza A virus.
- immunology : Influenza A virus, Peptides, T-Lymphocytes, Cytotoxic, Viral Proteins.
- prevention & control : Orthomyxoviridae Infections.
- Animals, Cross Reactions, Cytotoxicity, Immunologic, Female, Immunization, Lymphocyte Activation, Major Histocompatibility Complex, Mice, Mice, Inbred BALB C, Phenotype.
Abstract
We examined whether immunization with a single peptide induces cytotoxic T lymphocytes (CTLs) of heterogeneous specificities in vivo. Immunization of BALB/c mice with the peptide H2:529-537, which corresponded to amino acid residues 529-537 on the HA2 subunit transmembrane region of influenza A/Jap virus (H2N2) and possessed an H-2Kd-binding motif, induced CD8+CD4- CTLs. These CTLs lysed influenza A/Jap virus-infected target cells as well as those pulsed with the H2:529-537 peptide. H2:529-537 peptide-induced CTLs also lysed to lower but significant levels the target cells pulsed with the H1:533-541 peptide, which corresponded to amino acid residues 533-541 on the HA2 subunit transmembrane region of influenza A/PR/8 virus (H1N1) and were compatible to H2:529-537. Immunization with the H1:533-541 peptide, which also possessed an H-2Kd-binding motif, induced CTLs in vivo. H1:533-541-induced CTLs lysed influenza A/PR/8 virus-infected target cells and those pulsed with the peptide H1:533-541. Subtype cross-reactive CTLs to the H2:529-537 peptide were not induced by immunization with the H1:533-541 peptide. Two peptides, H2:3S and H2:7S, which had one amino acid substitution, serine at the third and seventh positions, respectively, induced CTLs that lysed target cells pulsed with the respective peptides to the highest levels. These results indicate that immunization with a single peptide induces CTLs of heterogeneous specificities in vivo.
DOI: 10.1089/vim.2000.13.73
PubMed: 10733170
Affiliations:
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Masaki</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, Kinki University School of Medicine, Osaka-sayama, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Microbiology, Kinki University School of Medicine, Osaka-sayama</wicri:regionArea><wicri:noRegion>Osaka-sayama</wicri:noRegion></affiliation></author><author><name sortKey="Tamura, M" sort="Tamura, M" uniqKey="Tamura M" first="M" last="Tamura">M. Tamura</name></author><author><name sortKey="Kurane, I" sort="Kurane, I" uniqKey="Kurane I" first="I" last="Kurane">I. Kurane</name></author></analytic><series><title level="j">Viral immunology</title><idno type="ISSN">0882-8245</idno><imprint><date when="2000" type="published">2000</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cross Reactions</term><term>Cytotoxicity, Immunologic</term><term>Female</term><term>Immunization</term><term>Influenza A virus (chemistry)</term><term>Influenza A virus (immunology)</term><term>Lymphocyte Activation</term><term>Major Histocompatibility Complex</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Orthomyxoviridae Infections (prevention & control)</term><term>Peptides (administration & dosage)</term><term>Peptides (immunology)</term><term>Phenotype</term><term>T-Lymphocytes, Cytotoxic (immunology)</term><term>Viral Proteins (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Activation des lymphocytes</term><term>Animaux</term><term>Complexe majeur d'histocompatibilité</term><term>Cytotoxicité immunologique</term><term>Femelle</term><term>Immunisation</term><term>Infections à Orthomyxoviridae ()</term><term>Lymphocytes T cytotoxiques (immunologie)</term><term>Peptides (administration et posologie)</term><term>Peptides (immunologie)</term><term>Phénotype</term><term>Protéines virales (immunologie)</term><term>Réactions croisées</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Virus de la grippe A ()</term><term>Virus de la grippe A (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Peptides</term></keywords><keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Peptides</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Lymphocytes T cytotoxiques</term><term>Peptides</term><term>Protéines virales</term><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A virus</term><term>Peptides</term><term>T-Lymphocytes, Cytotoxic</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Orthomyxoviridae Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cross Reactions</term><term>Cytotoxicity, Immunologic</term><term>Female</term><term>Immunization</term><term>Lymphocyte Activation</term><term>Major Histocompatibility Complex</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Phenotype</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Activation des lymphocytes</term><term>Animaux</term><term>Complexe majeur d'histocompatibilité</term><term>Cytotoxicité immunologique</term><term>Femelle</term><term>Immunisation</term><term>Infections à Orthomyxoviridae</term><term>Phénotype</term><term>Réactions croisées</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Virus de la grippe A</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We examined whether immunization with a single peptide induces cytotoxic T lymphocytes (CTLs) of heterogeneous specificities in vivo. Immunization of BALB/c mice with the peptide H2:529-537, which corresponded to amino acid residues 529-537 on the HA2 subunit transmembrane region of influenza A/Jap virus (H2N2) and possessed an H-2Kd-binding motif, induced CD8+CD4- CTLs. These CTLs lysed influenza A/Jap virus-infected target cells as well as those pulsed with the H2:529-537 peptide. H2:529-537 peptide-induced CTLs also lysed to lower but significant levels the target cells pulsed with the H1:533-541 peptide, which corresponded to amino acid residues 533-541 on the HA2 subunit transmembrane region of influenza A/PR/8 virus (H1N1) and were compatible to H2:529-537. Immunization with the H1:533-541 peptide, which also possessed an H-2Kd-binding motif, induced CTLs in vivo. H1:533-541-induced CTLs lysed influenza A/PR/8 virus-infected target cells and those pulsed with the peptide H1:533-541. Subtype cross-reactive CTLs to the H2:529-537 peptide were not induced by immunization with the H1:533-541 peptide. Two peptides, H2:3S and H2:7S, which had one amino acid substitution, serine at the third and seventh positions, respectively, induced CTLs that lysed target cells pulsed with the respective peptides to the highest levels. These results indicate that immunization with a single peptide induces CTLs of heterogeneous specificities in vivo.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><noCountry><name sortKey="Kurane, I" sort="Kurane, I" uniqKey="Kurane I" first="I" last="Kurane">I. Kurane</name><name sortKey="Tamura, M" sort="Tamura, M" uniqKey="Tamura M" first="M" last="Tamura">M. Tamura</name></noCountry><country name="Japon"><noRegion><name sortKey="Masaki, H" sort="Masaki, H" uniqKey="Masaki H" first="H" last="Masaki">H. Masaki</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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